Learn how PharmaFluidics µPAC TM cartridges overcome peak dispersion challenges of traditional packed fused silica columns with their proprietary flow distribution structures, resulting in less sample preparation, higher throughput and also an unprecedented analytical result.
Careful etching away the interstitial volumes out of a silicon wafer support leaves an array of pillars. This creates a stationary phase support structure that is organized in a reproducible, perfectly ordered pattern. The perfect order of the etched bed eliminates peak dispersion introduced by otherwise heterogeneous (eddy) flows in disordered/packed beds, creating much higher plate counts and sharper peaks. Consequently, samples dilute less during elution, increasing sensitivity.
Intrinsically free-standing, the pillar support structures allow flow with minimal back-pressure build up. Since there are no obstructions by touching surfaces of the stationary phase, blocking the regular flow of the mobile phase, µPACsTM return low back-pressures at high flow rates.
Since the separation bed is fixed to its silicon support, you can also use PharmaFluidics µPAC TM cartridges in reverse flow mode. This is a handy feature, enabling back flush in order to clean the column. Columns of unprecedented length (50 & 200 cm) are created on a small footprint. You can achieve this by interconnecting several separation lanes by means of proprietary turn structures. Carefully engineered flow distributors allow to make these turns with no additional peak dispersion. The resulting long columns give the sample more time to interact with the stationary phase, in order to create unmatched separation power.
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